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Heteronuclear NMR spectroscopy of proteins encapsulated in cubic phase lipids


Meikle, TG; Sethi, A; Keizer, DW; Babon, JJ; Separovic, F; Gooley, PR; Conn, CE; Yao, S
2019-07-01
Journal of Magnetic Resonance
Journal Article
305
146-151
Lipidic cubic phases, which form spontaneously via the self-assembly of certain lipids in an aqueous environment, are highly prospective nanomaterials with applications in membrane protein X-ray crystallography and drug delivery. Here we report (1)H-(15)N heteronuclear single/multiple quantum coherence (HSQC, HMQC) spectra of (15)N-enriched proteins encapsulated in inverse bicontinuous lipidic cubic phases obtained on a standard commercial high resolution NMR spectrometer at ambient temperature. (15)N-enriched proteins encapsulated in this lipidic cubic phase show: (i) no significant changes in tertiary structure, (ii) significantly reduced solvent chemical exchange of backbone amides, which potentially provides a novel concept for quantifying residue-specific hydration; and (iii) improved spectral sensitivity achieved with band-selective excitation short-transient (BEST) spectroscopy, which is attributed to the presence of an abundant source of (1)H nuclear spins originating from the lipid component of the cubic phase.
Elsevier
Structural Biology
10.1016/j.jmr.2019.06.017
31284168
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Creation Date 2019-07-17 01:31:56 Last Modified 2019-07-17 02:04:05