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Effects of modulators of Ca2+-activated, intermediate-conductance potassium channels on motility of the rat small intestine, in vivo


Ferens, D; Baell, J; Lessene, G; Smith, JE; Furness, JB
2007-05
NEUROGASTROENTEROLOGY AND MOTILITY
Journal Article
19
5
383-389
The movements of the intestine shift between different motor patterns, including between propulsion and mixing, but there is little information concerning mechanisms that may lead to changes in the patterns of motility. We have investigated the influence on intestinal motility of drugs that affect the after-hyperpolarization potential (AHP) of intrinsic primary afferent neurons (IPANs). The current of the AHP is carried by the intermediate conductance, calcium-activated, potassium (IK) channel. In anaesthetized rats, the IK channel blocker, 1-[(2-chlorophenyl)diphenylmethyl]-1H-pyrazole (0.05-1 mg kg(-1), i.v.) disrupted the regular propulsive pressure waves that occur in the small intestine and reduced propulsion of the contents (after 1 mg kg(-1), the fluid propelled was <25% of control). If the propulsion in the intestine was regular, the IK channel opener, 5,6-dichloro-1-ethyl-2-benzimidazolinone (DC-EBIO, 0.1 mg kg(-1) h(-1)) had no effect. DC-EBIO (0.1 mg kg(-1) h(-1)) restored propulsive activity after the nitric oxide synthase inhibitor, N omega-nitro-L-arginine had changed motility to a mixing pattern. We suggest that the AHP determines the synchrony of action potential firing in synaptically coupled IPANs, and that this synchrony influences the patterns of firing of muscle motor neurons, and hence the pattern of contraction of the muscle and whether the pattern is predominantly propulsive or predominantly mixing.
WILEY-BLACKWELL
MYENTERIC AH NEURONS; ENTERIC NERVOUS-SYSTEM; ACTIVITY-INDUCED EXPRESSION; PRIMARY AFFERENT NEURONS; PIG SMALL-INTESTINE; GUINEA-PIG; SUBMUCOSAL PLEXUS; ENHANCED EXCITABILITY; FOS IMMUNOREACTIVITY; EPITHELIAL-CELLS
10.1111/j.1365-2982.2007.00898.x
Refer to copyright notice on published article.

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Creation Date 2007-05-01 12:00:00