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Interaction of insulin-like growth factor (IGF)-I and -II with IGF binding protein-2: mapping the binding surfaces by nuclear magnetic resonance


Carrick, FE; Hinds, MG; McNeil, KA; Wallace, JC; Forbes, BE; Norton, RS
2005-06
JOURNAL OF MOLECULAR ENDOCRINOLOGY
Journal Article
34
3
685-698
The interaction of IGF binding protein-2 (IGFBP-2) with IGF-I and -II has been investigated in solution using nuclear magnetic resonance (NMR) spectroscopy. Chemical shift perturbations in (15)N- and (2)H/(15)N-labelled IGF-I or -II upon binding to unlabelled thioredoxin-tagged bovine IGFBP-2 (Trx(1-279)|GFBP-2) have been monitored to identify residues involved directly in the binding interaction as well as any affected by conformational changes associated with the interaction. A key step in obtaining high-quality spectra of the complexes was the use of transverse relaxation optimised spectroscopy (TROSY) methods with partially deuterated ligands. Indeed, because the effects of conformational averaging and aggregation are eliminated in IGF-I and -II bound to IGFBP-2, the spectra of the complexes are actually superior to those of the free ligands. Comparison of our results with the crystal structure of the complex between IGF-I and an N-terminal fragment of IGFBP-5 allowed identification of those residues perturbed by the C-domain of IGFBP-2. Other perturbations, such as those of Gly(19) and Asp(20) of IGF-I (and the corresponding residues in IGF-II) - which are located in a reverse turn linking N-domain and C-domain interactive surfaces - are due to local conformational changes in the IGF-I and -II. Our results confirm that the C-domain of IGFBP-2 plays a key role in binding regions of IGF-I and -II that are also involved in binding to the type-1 IGF receptor and thereby blocking ligand binding to this receptor.
BIOSCIENTIFICA LTD
LARGE BIOLOGICAL MACROMOLECULES; DISEASE ANTIGEN OSPA; FACTOR-I; SENSITIVITY ENHANCEMENT; SECONDARY STRUCTURE; NMR-SPECTROSCOPY; FUSION PROTEINS; TERMINAL DOMAIN; FACTOR RECEPTOR; B-DOMAIN
10.1677/jme.1.01756
Refer to copyright notice on published article.

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Creation Date 2005-06-01 12:00:00