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Defective neural tube closure and anteroposterior patterning in mice lacking the LIM protein LMO4 or its interacting partner deaf-1


Hahm, KM; Sum, EYM; Fujiwara, Y; Lindeman, GJ; Visvader, JE; Orkin, SH
2004-03
MOLECULAR AND CELLULAR BIOLOGY
Journal Article
24
5
2074-2082
LMO4 belongs to a family of transcriptional regulators that comprises two zinc-binding LIM domains. LIM-only (LMO) proteins appear to function as docking sites for other factors, leading to the assembly of multiprotein complexes. The transcription factor Deaf-1/NUDR has been identified as one partner protein of LMO4. We have disrupted the Lmo4 and Deaf-1 genes in mice to define their biological function in vivo. All Lmo4 mutants died shortly after birth and showed defects within the presphenoid bone, with 50% of mice also exhibiting exencephaly. Homeotic transformations were observed in Lmo4-null embryos and newborn mice, but with incomplete penetrance. These included skeletal defects in cervical vertebrae and the rib cage. Furthermore, fusions of cranial nerves IX and X and defects in cranial nerve V were apparent in some Lmo4(-/-) and Lmo4(+/-) mice. Remarkably, Deaf-1 mutants displayed phenotypic abnormalities similar to those observed in Lmo4 mutants. These included exencephaly, transformation of cervical segments, and rib cage abnormalities. In contrast to Lmo4 nullizygous mice, nonexencephalic Deaf-1 mutants remained healthy. No defects in the sphenoid bone or cranial nerves were apparent. Thus, Lmo4 and Deaf-1 mutant mice exhibit overlapping as well as distinct phenotypes. Our data indicate an important role for these two transcriptional regulators in pathways affecting neural tube closure and skeletal patterning, most likely reflecting their presence in a functional complex in vivo.
AMER SOC MICROBIOLOGY
ONLY PROTEIN; HOMEOTIC TRANSFORMATIONS; HOMEODOMAIN PROTEINS; TRANSCRIPTION FACTOR; MOLECULAR-CLONING; CRE RECOMBINASE; SAND DOMAIN; GENE; EXPRESSION; IDENTIFICATION
10.1128/MCB.24.5.2074-2082.2004
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Creation Date 2004-03-01 12:00:00