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Report from the 1st International NOD Mouse T-Cell Workshop and the follow-up mini-workshop


Kaufman, DL; Tisch, R; Sarvetnick, N; Chatenoud, L; Harrison, LC; Haskins, K; Quinn, A; Sercarz, E; Singh, B; von Herrath, M; Wegmann, D; Wen, L; Zekzer, D
2001-11
DIABETES
Journal Article
50
11
2459-2463
A workshop on autoreactive T-cell responses in NOD mice was held to optimize autoreactive T-cell detection methodologies. Using different proliferation assay protocols, 1 of the 11 participating laboratories detected spontaneous T-cell responses to GAD(524-543) and insulin(9-23) in their NOD mice. Two other laboratories were able to detect autoreactive responses when using enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA) analysis of cytokines in culture supernatants, suggesting that these assays provided greater sensitivity. To address the divergent findings, a follow-up mini-workshop tested NOD mice from four different colonies side-by-side for T-cell proliferative responses to an expanded panel of autoantigens, using the protocol that had enabled detection of responses in the 1st International NOD Mouse T-Cell Workshop. Under these assay conditions, 16 of 16 NOD mice displayed proliferative responses to whole GAD65, 13 of 16 to GAD(524-543), 9 of 16 to GAD(217-236), 7 of 16 to insulin(9-23), and 5 of 16 to HSP277. Thus, spontaneous proliferative T-cell responses can be consistently detected to some P-cell autoantigens and peptides thereof. Overall, the results suggest that more sensitive assays (e.g., ELISPOT, ELISA analysis of cytokines in supernatants, or tetramer staining) may be preferred for the detection of autoreactive T-cells.
AMER DIABETES ASSOC
NONOBESE DIABETIC MICE; INSULIN
10.2337/diabetes.50.11.2459
Refer to copyright notice on published article.

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Creation Date 2001-11-01 12:00:00