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Mouse LIM-kinase 2 gene: cDNA cloning, genomic organization, and tissue-specific expression of two alternatively initiated transcripts


Ikebe, C; Ohashi, K; Fujimori, T; Bernard, O; Noda, T; Robertson, EJ; Mizuno, K
1997-12-15
GENOMICS
Journal Article
46
3
504-508
LIM-kinase 1 and LIM-kinase 2 (LIMK1 and LIMK2) are members of a novel protein kinase subfamily containing LIM motifs at the N-terminus, There are two isoforms of Limk2 transcripts coding proteins with distinct N-terminal structures: LIMK2a, containing two LIM motifs, and LIMK2b, with one and one-half LIM motifs. Here we report the cDNA and genomic structures of mouse LIMK2. The deduced 638-amino-acid sequence of mouse LIMK2a shows 98% identity with that of rat LIMK2a. The mouse Limk2a gene consists of at least 16 exons and spans more than 50 kb. Exon/intron boundaries of the mouse Limk2a gene are exactly conserved with those of the mouse Limk1 gene. An additional exon encoding the Limk2b-specific 5'-terminal sequence was found to be located between exons 2 and 3, suggesting that Limk2a and 2b mRNAs are transcribed from a single Limk2 gene by an alternative usage of exons near the 5' end of the gene. Limk2a and Limk2b transcripts were expressed at different ratios in a variety of mouse tissues. (C) 1997 Academic Press.
ACADEMIC PRESS INC
PROTEIN-KINASE; ZINC-FINGER; THREONINE; SERINE; IDENTIFICATION; FAMILY; MOTIFS
10.1006/geno.1997.5060
Refer to copyright notice on published article.

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Creation Date 1997-12-15 12:00:00