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NSE-bcl-2 transgenic mice, a model system for studying neuronal death and survival


Bernard, R; Farlie, P; Bernard, O
1997-01
DEVELOPMENTAL NEUROSCIENCE
Journal Article
19
1
79-85
Bcl-2 is a crucial regulator of cell survival and death. We have recently demonstrated that transgenic mice overexpressing the human Bcl-2 protein specifically in their neurons have an increased number of neuronal cells which can survive in tissue culture in the absence of neurotrophic factors. In order to understand why only some neurons can be rescued from developmental and neurotrophic factor deprivation-induced death, we have studied the expression pattern of the transgene during embryonic development and in adulthood. We have demonstrated that transgene expression starts in embryos at E12.5 and that only half of the sensory neurons of the dorsal root ganglia expressed detectable levels of the human Bcl-2. These results may explain why only 40% of the sensory neurons survived in tissue culture in the absence of neurotrophic factors.
KARGER
PROGRAMMED CELL-DEATH; BCL-2 HOMOLOG BAK; CAENORHABDITIS-ELEGANS; IL-1-BETA-CONVERTING ENZYME; PROTOONCOGENE BCL-2; NERVOUS-SYSTEM; GENE CED-3; IN-VIVO; APOPTOSIS; PROTEIN
10.1159/000111188
Refer to copyright notice on published article.

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Creation Date 1997-01-01 12:00:00