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Photoaffinity labeling of mefloquine-binding proteins in human serum, uninfected erythrocytes sind Plasmodium falciparum-infected erythrocytes


Desneves, J; Thorn, G; Berman, A; Galatis, D; LaGreca, N; Sinding, J; Foley, M; Deady, LW; Cowman, AF; Tilley, L
1996-11-25
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Journal Article
82
2
181-194
A photoreactive quinolinemethanol analog, N-[4-[1-hydroxy-2-(dibutylamino)ethyl]quinolin-8-yl]-4-azido-2-salicylamide (ASA-MQ) has been synthesized which closely mimics the action of mefloquine. ASA-MQ possesses potent antimalarial activity against a mefloquine-sensitive strain of Plasmodium falciparum and shows decreased activity against a mefloquine-resistant parasite strain. Radioiodinated ASA-MQ has been used in photoaffinity labeling studies to identify mefloquine-interacting proteins in serum, uninfected erythrocytes and Plasmodium falciparum-infected erythrocytes. We have shown that mefloquine interacts specifically with ape-Al, the major protein of serum high density lipoproteins. In addition, mefloquine was shown to interact specifically with the erythrocyte membrane protein, band 7.2b (stomatin). A further two high affinity mefloquine-binding proteins with apparent molecular masses of 22 and 36 kDa were identified in three different strains of Plasmodium falciparum. We suggest that these two mefloquine-binding parasite proteins may be involved in the uptake of mefloquine or may represent macromolecular targets of mefloquine action in malaria parasites.
ELSEVIER SCIENCE BV
HIGH-DENSITY-LIPOPROTEINS; MALARIA PARASITES; HEME POLYMERASE; PFMDR1 GENE; CHLOROQUINE; AMPLIFICATION; RESISTANCE; HALOFANTRINE; DRUG; IDENTIFICATION
10.1016/0166-6851(96)02732-6
Refer to copyright notice on published article.

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Creation Date 1996-11-25 12:00:00