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Plasmodium falciparum: Amplification and overexpression of pfmdr1 is not necessary for increased mefloquine resistance


Lim, ASY; Galatis, D; Cowman, AF
1996-08
EXPERIMENTAL PARASITOLOGY
Journal Article
83
3
295-303
Amplification and overexpression of the pfmdr1 gene has been associated with mefloquine resistance in Plasmodium falciparum. We have selected for parasites more resistant to mefloquine from P. falciparum FAC8, a clone that has three copies of pfmdr1. The parasite lines derived from this selection were up to threefold more resistant to mefloquine. The mefloquine-selected parasites were also more resistant to quinine and halofantrine, suggesting a multidrug resistance phenotype. In contrast to previous findings, the selection of P. falciparum FAGS on mefloquine did not alter the copy number or the level of expression of pfmdr1. Sequence analysis of pfmdr1 from the selected lines showed no change in the amino acids. These results show that alterations in pfmdr1 are not involved in mediating the increased mefloquine resistance observed in this clone. (C) 1996 Academic Press, Inc.
ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
P-GLYCOPROTEIN HOMOLOG; CHLOROQUINE-RESISTANCE; SULFADOXINE-PYRIMETHAMINE; HALOFANTRINE RESISTANCE; MALARIA PARASITES; GENE; THAILAND; DNA; SEPARATION; SELECTION
10.1006/expr.1996.0077
Refer to copyright notice on published article.

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Creation Date 1996-08-01 12:00:00