Research Publications

Back

Complex of the soluble IL-11 receptor and IL-11 acts as IL-6-type cytokine in hepatic and nonhepatic cells


Baumann, H; Wang, YP; Morella, KK; Lai, CF; Dams, H; Hilton, DJ; Hawley, RG; Mackiewicz, A
1996-07-01
JOURNAL OF IMMUNOLOGY
Journal Article
157
1
284-290
The signaling functions of the membrane and soluble form of the mouse IL-11 receptor (mIL-11R) were compared in rat and human hepatoma cells, which have a low endogenous IL-11 response. The expression vectors encoding either the full length or a secretory form of the ligand binding subunit of mIL-11R together with IL-6-responsive reporter gene constructs were transiently transfected into the H-35 and HepG2, cells. An IL-11-specific stimulation of transcription was detected that was qualitatively similar to that mediated by the endogenous IL-6R. HepG2 cells were noted to synthesize constitutively IL-11, resulting in an autocrine stimulation of gene expression. Addition of COS cell-derived soluble mIL-11R to the hepatoma cell cultures prominently enhanced IL-11 regulation of transfected reporter gene constructs and expression of endogenous acute phase plasma protein genes. Similarly, the complex of soluble mIL-11R and IL-11 was capable of mediating an IL-6-type signaling in cells that are naturally deficient in It-ii response as shown by the activation of STAT1 and STAT3 in mouse embryonal carcinoma cells and human T cells. The results indicate that the IL-11R can serve as a substitute to IL-6R in activating gene expression in target cells that are devoid of the appropriate ligand-binding receptor subunits.
AMER ASSOC IMMUNOLOGISTS
INTERLEUKIN-6 RECEPTOR; SIGNAL-TRANSDUCTION; EXPRESSION; PROTEINS; IDENTIFICATION; INVIVO; GP130; SERUM; GENE; ACID
Refer to copyright notice on published article.

Back
Creation Date 1996-07-01 12:00:00