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Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors


Uren, AG; Pakusch, M; Hawkins, CJ; Puls, KL; Vaux, DL
1996-05-14
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Journal Article
93
10
4974-4978
Baculovirus inhibitors of apoptosis (IAPs) act in insect cells to prevent cell death, Here we describe three mammalian homologs of IAP, MIHA, MIHB, and MIHC, and a Drosophila IAP homolog, DIHA. Each protein bears three baculovirus IAP repeats and an N-terminal ring finger motif. Apoptosis mediated by interleukin 1 beta converting enzyme (ICE), which can be inhibited by Orgyia pseudotsugata nuclear polyhedrosis virus IAP (OpIAP) and compos virus crmA, was also inhibited by MIHA and MIHB. As MIHB and MIHC were able to bind to the tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 in yeast two-hybrid assays, these results suggest that IAP proteins that inhibit apoptosis may do so by regulating signals required for activation of ICE-like proteases.
NATL ACAD SCIENCES
PROGRAMMED CELL-DEATH; BACULOVIRUS GENE; INSECT CELLS; C-ELEGANS; PREVENTION; SURVIVAL; ENZYME; BCL-2
10.1073/pnas.93.10.4974
Refer to copyright notice on published article.

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Creation Date 1996-05-14 12:00:00