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NERVE GROWTH FACTOR-REGULATED PROPERTIES OF SENSORY NEURONS IN OCT-2 NULL MUTANT MICE


NINKINA, NN; BUCHMAN, VL; AKOPIAN, AN; LAWSON, SN; Yamamoto, M; CAMPBELL, E; Corcoran, L; WOOD, JN
1995-11
MOLECULAR BRAIN RESEARCH
Journal Article
33
2
233-244
The POU-domain transcription factor Oct-2 is expressed in both B lymphocytes and sensory neurones, where its expression is regulated by nerve growth factor (NGF), In order to define a possible role for Oct-2 in neurotrophin signalling, we examined the expression of an NGF-regulated channel (capsaicin-evoked ion fluxes), neuropeptides (substance P, calcitonin gene-related peptide), structural proteins (neurofilaments and peripherin) and receptors (trks) in dorsal root ganglion neurones derived from perinatal transgenic mice containing a defective Oct-2 structural gene. Northern blots show that central nervous tissue contains a larger than normal(> 10 kb) mRNA transcript corresponding in size to an Oct-2 transcript encoding a defective protein. PCR analysis shows the absence of normal Oct-2 transcripts in dorsal root ganglia. In null mutants, capsaicin sensitivity, and neuropeptide and cytoskeletal protein expression were unaffected by the loss of Oct-2 expression. The number of sensory neurones and the gross morphology of CNS tissues that normally express high levels of Oct-2 were also examined and found to be normal in the null mutant. Heterozygous animals show normal thresholds of sensitivity to noxious heat and normal inflammatory responses. Oct-2 does not therefore play an essential role in the NGF responsiveness of sensory neurones in these animals.
ELSEVIER SCIENCE BV
TRANSCRIPTION FACTORS; B-CELL; PROTEIN OCT-2; GENE-PRODUCTS; HOMEO DOMAIN; POU-DOMAIN; CGRP GENE; ADULT-RAT; EXPRESSION; DIFFERENTIATION
10.1016/0169-328X(95)00128-F
Refer to copyright notice on published article.

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Creation Date 1995-11-01 12:00:00