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Adult Cardiac-Resident MSC-like Stem Cells with a Proepicardial Origin


Chong, JJH; Chandrakanthan, V; Xaymardan, M; Asli, NS; Li, J; Ahmed, I; Heffernan, C; Menon, MK; Scarlett, CJ; Rashidianfar, A; Biben, C; Zoellner, H; Colvin, EK; Pimanda, JE; Biankin, AV; Zhou, B; Pu, WT; Prall, OWJ; Harvey, RP
2011-12-02
CELL STEM CELL
Journal Article
9
6
527-540
Colony-forming units fibroblast (CFU-Fs), analogous to those giving rise to bone marrow (BM) mesenchymal stem cells (MSCs), are present in many organs, although the relationship between BM and organ-specific CFU-Fs in homeostasis and tissue repair is unknown. Here we describe a population of adult cardiac-resident CFU-Fs (cCFU-Fs) that occupy a perk vascular, adventitial niche and show broad trans-germ layer potency in vitro and in vivo. CRE lineage tracing and embryo analysis demonstrated a proepicardial origin for cCFU-Fs. Furthermore, in BM transplantation chimeras, we found no interchange between BM and cCFU-Fs after aging, myocardial infarction, or BM stem cell mobilization. BM and cardiac and aortic CFU-Fs had distinct CRE lineage signatures, indicating that they arise from different progenitor beds during development. These diverse origins for CFU-Fs suggest an underlying basis for differentiation biases seen in different CFU-F populations, and could also influence their capacity for participating in tissue repair.
CELL PRESS
CORONARY-ARTERY FORMATION; NEURAL CREST CELLS; BONE-MARROW; PROGENITOR CELLS; C-KIT; PERIPHERAL-BLOOD; MAMMALIAN HEART; IN-VITRO; CFU-F; INJURY
10.1016/j.stem.2011.10.002
Copyright © 2011 Elsevier Inc. All rights reserved.