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NUCLEOTIDE BINDING-PROPERTIES OF A P-GLYCOPROTEIN HOMOLOG FROM PLASMODIUM-FALCIPARUM


KARCZ, SR; Galatis, D; Cowman, AF
1993-04
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Journal Article
58
2
269-276
The Plasmodium falciparum P-glycoprotein homologue 1 (PGH1) is structurally similar to several members of the ATP-binding cassette (ABC) superfamily of membrane transporters. We have examined whether the nucleotide binding domains predicted from the deduced amino sequence are functional by photoaffinity labeling of purified parasite digestive vacuoles with the analogue 8-azido-alpha-[P-32]ATP (8-N3-ATP). This reagent labels a 160-kDa protein in vacuoles from both a chloroquine sensitive and a chloroquine-resistant parasite isolate. The 160-kDa protein could be immunoprecipitated with affinity-purified antibodies against the P. falciparum P-glycoprotein homologue (PGH1). Inhibition of photoaffinity labeling of PGH1 could be achieved with ATP, ADP, GTP and GDP but not with AMP or GMP. In order to map the 8-N3-ATP binding sites on PGH1, photoaffinity-labeled PGH1 was digested with trypsin and immunoprecipitated with site-specific antibodies. Taken together, these results indicate that 8-N3-ATP specifically labels PGH1 and that one binding site resides within the amino terminal half of the molecule. This supports the contention that PGH1 is involved in a nucleotide-regulated transport function across the membrane of the digestive vacuole.
ELSEVIER SCIENCE BV
MULTIDRUG-RESISTANCE GENE; CHLOROQUINE-RESISTANCE; BACTERIAL TRANSPORT; ESCHERICHIA-COLI; PROTEINS; CELLS; AMPLIFICATION; SECRETION; MUTATIONS; SITES
10.1016/0166-6851(93)90048-3
Refer to copyright notice on published article.

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Creation Date 1993-04-01 12:00:00