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ELIMINATION OF SELF-REACTIVE LYMPHOCYTES-B PROCEEDS IN 2 STAGES - ARRESTED DEVELOPMENT AND CELL-DEATH


HARTLEY, SB; COOKE, MP; FULCHER, DA; Harris, AW; Cory, S; Basten, A; Goodnow, CC
1993-02-12
CELL
Journal Article
72
3
325-335
In transgenic mice, self-reactive B lymphocytes are eliminated if they encounter membrane-bound self antigens during their development within the bone marrow. We show here that two separate and sequential events, arrested development and cell death, bring about B cell elimination. Developmental arrest is an early outcome of antigen binding in immature B cells, blocks acquisition of adhesion molecules and receptors important for B cell migration and activation, and is rapidly reversible by removal of antigen. Death of the arrested B cells occurs within 1 to 3 days and can be delayed by expression of a bcl-2 transgene, which results in escape of large numbers of self-reactive B cells from the bone marrow but fails to override the developmental arrest. These findings define a novel pathway for B cell elimination, involving an initial stage vulnerable to breakdown in autoimmune disease.
CELL PRESS
COMPLEMENT RECEPTOR TYPE-2; IMMUNOGLOBULIN MU-CHAIN; MOUSE BONE-MARROW; TRANSGENIC MICE; CLONAL DELETION; ANTIBODY-RESPONSES; T-CELLS; EXPRESSION; TOLERANCE; DIFFERENTIATION
10.1016/0092-8674(93)90111-3
Refer to copyright notice on published article.

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Creation Date 1993-02-12 12:00:00