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ENHANCED INSULIN-RECEPTOR TYROSINE KINASE-ACTIVITY ASSOCIATED WITH CHROMOSOMAL TRANSLOCATION (1-19) IN A PRE-B-CELL LEUKEMIA LINE


NEWMAN, JD; Harrison, LC; ECKARDT, GS; JACK, I
1992-02-01
INTERNATIONAL JOURNAL OF CANCER
Journal Article
50
3
500-504
The gene for the insulin receptor has been assigned to chromosome 19 near the breakpoint of the translocation t(1;19) which occurs in 25% of pre-B-cell leukemias. Insulin receptors in a pre-B-cell leukemia cell line (ACV) with t(1;19) were found to have 2-fold higher affinity for insulin, 5-fold higher basal and insulin-stimulated beta-sub-unit autophosphorylation, and 2-fold higher basal and 4-fold higher insulin-stimulated beta-sub-unit kinase activity on the synthetic peptide poly(Glu,Tyr), compared to receptors in a B-cell line (ADD) with normal karyotype from the same patient. ACV cells had a novel 13-kb receptor mRNA species and expressed a DNA polymorphism localized to the tyrosine kinase domain of the receptor gene. These findings suggest that t(1;19) in the ACV cell may result in rearrangement of the insulin receptor gene and translation of a receptor with enhanced tyrosine kinase activity.
WILEY-LISS
LYMPHOBLASTIC-LEUKEMIA; GENE; EXPRESSION; ONCOGENES; PROTEIN; BREAKPOINT; BINDING; FAMILY; BCR
10.1002/ijc.2910500328
Refer to copyright notice on published article.

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Creation Date 1992-02-01 12:00:00