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TIMING OF DELETION OF AUTOREACTIVE V-BETA-6+ CELLS AND DOWN-MODULATION OF EITHER CD4 OR CD8 ON PHENOTYPICALLY DISTINCT CD4+8+ SUBSETS OF THYMOCYTES EXPRESSING INTERMEDIATE OR HIGH-LEVELS OF T-CELL RECEPTOR


HUGO, P; Boyd, RL; WAANDERS, GA; PETRIE, HT; Scollay, R
1991-03
INTERNATIONAL IMMUNOLOGY
Journal Article
3
3
265-272
In this paper we describe a differentiation sequence amongst adult murine thymocytes which goes from CD4+8+3lo(low) to CD4+8+3int(intermediate) to CD4+8+3hi(high) and then to mature single positive CD3hi thymocytes. Phenotypic characterization of CD4+8+3int/hi cells for a number of other surface markers is consistent with them being in transition from CD4+8+3lo phenotype to mature phenotype. The same observation was made for sensitivity towards ionomycin-mediated apoptosis. In the thymus of Mls-1a mice, where autoreactive TCR-V-beta-6+ cells are negatively selected, deletion of TCR-V-beta-6+ cells was first detected in the CD4+8+3int subset, and was complete by the CD4+8+3hi stage, suggesting that up-regulation of the TCR/CD3 complex is required for deletion of Mls-1a autoreactive thymocytes. No sign of apoptosis was detected among any fresh thymocyte subsets suggesting that apoptotic cells are rapidly cleared from the thymus. The CD4+8+3int/CD4+8+3hi cells are therefore populations in transit from the typical cortical thymocytes to the mature T-cells.
OXFORD UNIV PRESS UNITED KINGDOM
MLSA-ENCODED ANTIGENS; MAJOR HISTOCOMPATIBILITY COMPLEX; CHAIN TRANSGENIC MICE; NEGATIVE SELECTION; MURINE THYMOCYTES; LYMPHOCYTES-T; TOLERANCE; THYMUS; APOPTOSIS; DEATH
10.1093/intimm/3.3.265
Refer to copyright notice on published article.

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Creation Date 1991-03-01 12:00:00