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ALPHA-B CRYSTALLIN ACCUMULATION IS A SPECIFIC RESPONSE TO HA-RAS AND V-MOS ONCOGENE EXPRESSION IN MOUSE NIH 3T3 FIBROBLASTS


KLEMENZ, R; FROHLI, E; AOYAMA, A; HOFFMANN, S; Simpson, RJ; Moritz, RL; SCHAFER, R
1991-02
MOLECULAR AND CELLULAR BIOLOGY
Journal Article
11
2
803-812
The conditional expression of the v-mos and Ha-ras(EJ) oncogenes in NIH 3T3 cells leads to the accumulation of a 23-kDa protein (p23) (R. Klemenz, S. Hoffmann, R. Jaggi, and A.-K. Werenskiold, Oncogene 4:799-803, 1989). We purified p23 to homogeneity and determined part of the amino acid sequence. The obtained sequence is identical with that of the eye lens protein alpha-B crystallin. Northern (RNA) blot and Western immunoblot experiments were performed to demonstrate that alpha-B crystallin mRNA and protein do indeed accumulate as a consequence of v-mos and Ha-ras oncogene expression. Comparison of cDNA clones obtained from the mRNA of eye lenses and of oncogene-expressing fibroblasts revealed identity between them. The major transcription initiation site of the alpha-B crystallin gene in our experimental system was shown by primer extension experiments to be identical with one used in eye epithelial cells. In addition, we identified a second minor initiation site 49 nucleotides further upstream. Serum growth factors did not stimulate alpha-B crystallin expression in growth-arrested cells.
AMER SOC MICROBIOLOGY
HEAT-SHOCK PROTEIN; RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS; EPIDERMAL GROWTH-FACTOR; AMINO-ACID SEQUENCE; B-CRYSTALLIN; LENS CRYSTALLINS; GENE-EXPRESSION; POLYACRYLAMIDE GELS; TRANSFORMED-CELLS; TRANSGENIC MICE
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Creation Date 1991-02-01 12:00:00