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GM-CSF increases cross-presentation and CD103 expression by mouse CD8(+) spleen dendritic cells


Zhan, YF; Carrington, EM; van Nieuwenhuijze, A; Bedoui, S; Seah, S; Xu, YK; Wang, N; Mintern, JD; Villadangos, JA; Wicks, IP; Lew, AM
2011-09
EUROPEAN JOURNAL OF IMMUNOLOGY
Journal Article
41
9
2585-2595
Resident CD8(+) DCs perform several functions, including cross-presenting antigen and rapidly engulfing the Gram-positive intracellular pathogen Listeria monocytogenes. Little is known about how these functions of CD8(+) DCs are modulated. Here, we show that granulocyte-macrophage CSF (GM-CSF), a cytokine that exists at low levels at steady state but is elevated during infection and inflammation, enhances cross-presentation and rapid uptake of L. monocytogenes by resident CD8(+) DCs. This previously unrecognized functional enhancement of CD8(+) DCs by GM-CSF was independent of promoting DC survival in vitro. Enhancement of these functions by GM-CSF was also marked by CD103 expression on CD8(+) DCs that was strongly regulated by GM-CSF. Our findings not only identify GM-CSF as a key molecule regulating CD8(+) DC function, but also as a factor responsible for functional heterogeneity of CD8(+) DCs that is at least substantially demarcated by CD103 expression.
WILEY-BLACKWELL
COLONY-STIMULATING FACTOR; IN-VIVO; T-CELLS; LISTERIA-MONOCYTOGENES; EXOGENOUS ANTIGENS; BONE-MARROW; FLT3 LIGAND; SUBSET; MICE; COMPARTMENT
10.1002/eji.201141540
Refer to copyright notice on published article.