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Disruption of the Plasmodium falciparum liver-stage antigen-1 locus causes a differentiation defect in late liver-stage parasites


Mikolajczak, SA; Sacci, JB; De La Vega, P; Camargo, N; VanBuskirk, K; Krzych, U; Cao, J; Jacobs-Lorena, M; Cowman, AF; Kappe, SHI
2011-08
CELLULAR MICROBIOLOGY
Journal Article
13
8
1250-1260
The malaria parasite Plasmodium falciparum infects humans and first targets the liver where liver-stage parasites undergo pre-erythrocytic replication. Liver-stage antigen-1 (LSA-1) is currently the only identified P. falciparum protein for which expression is restricted to liver stages. Yet, the importance of LSA-1 for liver-stage parasite development remains unknown. Here we deleted LSA-1 in the NF54 strain of P. falciparum and analysed the lsa-1(-) parasites throughout their life cycle. lsa-1(-) sporozoites had normal gliding motility and invasion into hepatocytes. Six days after infection of a hepatocytic cell line, lsa-1-parasites exhibited a moderate phenotype with an similar to 50% reduction of late liver-stage forms when compared with wild type. Strikingly, lsa-1-parasites growing in SCID/Alb-uPA mice with humanized livers showed a severe defect in late liver-stage differentiation and exo-erythrocytic merozoite formation 7 days after infection, a time point when wild-type parasites develop into mature merozoites. The lsa-1(-) parasites also showed aberrant liver-stage expression of key parasite proteins apical membrane antigen-1 and circumsporozoite protein. Our data show that LSA-1 plays a critical role during late liver-stage schizogony and is thus important in the parasite transition from the liver to blood. LSA-1 is the first P. falciparum protein identified to be required for this transitional stage of the parasite life cycle.
WILEY-BLACKWELL
CHIMERIC HUMAN LIVERS; MALARIA PARASITES; SPOROZOITES; INFECTION; ESTABLISHMENT; TRANSMISSION; HEPATOCYTES; PROTECTION; RESPONSES; PROTEINS
10.1111/j.1462-5822.2011.01617.x
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