Research Publications

Back

Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion


Hanssen, E; Dekiwadia, C; Riglar, DT; Rug, M; Lemgruber, L; Cowman, AF; Cyrklaff, M; Kudryashev, M; Frischknecht, F; Baum, J; Ralph, SA
2013-09
CELLULAR MICROBIOLOGY
Journal Article
15
9
1457-1472
Erythrocyte invasion by merozoites forms of the malaria parasite is a key step in the establishment of human malaria disease. To date, efforts to understand cellular events underpinning entry have been limited to insights from non-human parasites, with no studies at sub-micrometer resolution undertaken using the most virulent human malaria parasite, Plasmodium falciparum. This leaves our understanding of the dynamics of merozoite sub-cellular compartments during infection incomplete, in particular that of the secretory organelles. Using advances in P. falciparum merozoite isolation and new imaging techniques we present a three-dimensional study of invasion using electron microscopy, cryo-electron tomography and cryo-X-ray tomography. We describe the core architectural features of invasion and identify fusion between rhoptries at the commencement of invasion as a hitherto overlooked event that likely provides a critical step that initiates entry. Given the centrality of merozoite organelle proteins to vaccine development, these insights provide a mechanistic framework to understand therapeutic strategies targeted towards the cellular events of invasion.
WILEY-BLACKWELL
RED-BLOOD-CELLS; MALARIA PARASITE INVASION; TOXOPLASMA-GONDII; APICOMPLEXAN PARASITES; INFECTED ERYTHROCYTES; FINE STRUCTURE; SUBPELLICULAR MICROTUBULES; RHOPTRY PROTEINS; SURFACE-ANTIGEN; DENSE GRANULES
Infection and Immunity
10.1111/cmi.12132
© 2013 John Wiley & Sons Ltd

Back
Creation Date 2013-09-01 12:00:00