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Resident and Monocyte-Derived Dendritic Cells Become Dominant IL-12 Producers under Different Conditions and Signaling Pathways


Zhan, YF; Xu, YK; Seah, S; Brady, JL; Carrington, EM; Cheers, C; Croker, BA; Wu, L; Villadangos, JA; Lew, AM
2010-08-15
JOURNAL OF IMMUNOLOGY
Journal Article
185
4
2125-2133
IL-12 is such a pivotal cytokine that it has been called the third signal for T cell activation, TCR engagement being the first and costimulation being the second. It has been generally viewed that the resident CD8(+) dendritic cell (DC) subset is the predominant IL-12-producing cell type. In this study, we found, although this is so under steady state conditions, under inflammatory conditions monocyte-derived DC (mDC) became a major cell type producing IL-12. Depletion of either type of DC resulted in reduced production of IL-12 in vivo. For CD8(+) DC, IL-12 production could be stimulated by various pathways viz. signaling through MyD88, Trif, or nucleotide-binding oligomerization domain (Nod)-like receptors. In contrast, for mDC, IL-12 production was mainly dependent on MyD88 signaling. Thus, conventional DCs and mDCs use different pathways to regulate IL-12 production. The Journal of Immunology, 2010, 185: 2125-2133.
AMER ASSOC IMMUNOLOGISTS
COLONY-STIMULATING FACTOR; TUMOR-NECROSIS-FACTOR; IN-VIVO; LISTERIA-MONOCYTOGENES; FLT3 LIGAND; T-CELLS; BACTERIAL-INFECTION; CROSS-PRESENTATION; ADAPTIVE IMMUNITY; RECEPTOR LIGANDS
10.4049/jimmunol.0903793
Refer to copyright notice on published article.