CD8(+) T cell help is required for efficient induction of EAE in Lewis rats
Camara, M; Beyersdorf, N; Fischer, HJ; Herold, MJ; Ip, CW; van den Brandt, J; Toyka, KV; Taurog, JD; Hunig, T; Herrmann, T; Reichardt, HM; Weishaupt, A; Kerkau, T
JOURNAL OF NEUROIMMUNOLOGY
The role of CD8(+) T cells in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE) is still unclear. We describe here significantly reduced disease activity of EAE both in Lewis rats, depleted of CD8(+) T cells by monoclonal antibodies and CD8 knockout rats, which was accompanied by reduced leukocyte infiltration into the spinal cord. We detected myelin basic protein (MBP)-specific CD4(+) T cells in peripheral lymphoid organs of CD8-depleted animals which, however, failed to differentiate into interferon-gamma-producing effector cells. Our results indicate that CD8(+) T cells interact with myelin-specific CD4(+) T cells early in EAE enabling them to differentiate into pathogenic effector cells. (C) 2013 Elsevier B.V. All rights reserved.