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Lineage tracing of mammary stem and progenitor cells


van de Moosdijk, AA; Fu, NY; Rios, AC; Visvader, JE; van Amerongen, R
2017
Methods Mol Biol
Journal Article
1501
291-308
Lineage tracing analysis allows mammary epithelial cells to be tracked in their natural environment, thereby revealing cell fate and proliferation choices in the intact tissue. This technique is particularly informative for studying how stem cells build and maintain the mammary epithelium during development and pregnancy. Here we describe two experimental systems based on Cre/loxP technology (CreERT2/loxP and rtTA/tetO-Cre/loxP), which allow the inducible, permanent labeling of mammary epithelial cells following the administration of either tamoxifen or doxycycline.
Springer
Stem Cells and Cancer
10.1007/978-1-4939-6475-8_15
27796960
Refer to copyright notice on published article.

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Creation Date 2017-05-26 03:11:45 Last Modified 2017-05-26 03:47:24