The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice
- Author(s)
- Martin, RM; Brady, JL; Lew, AM;
- Details
- Publication Year 1998-03-15,Volume 212,Issue #2,Page 187-192
- Journal Title
- JOURNAL OF IMMUNOLOGICAL METHODS
- Publication Type
- Journal Article
- Abstract
- Isotyping and quantitation of murine IgG2a antibodies are widely performed with commercial monoclonal and polyclonal antisera raised against BALB/c IgG2a myeloma proteins. Recently it became evident that inbred mouse strains with the Ighl-b allele do not have the gene for IgG2a and instead express the IgG2c isotype. We show that commercial anti-IgG2a sera cross-react inadequately against IgG2c in immunoblot and ELISA and hence, are not suitable to detect and measure this subclass in mouse strains such as C57BL/6, C57BL/10 and NOD. We have used DNA immunization to generate polyclonal anti-IgG2c serum and demonstrated that it is essential to use IgG2c-specific antiserum to quantify accurately isotypic responses in mouse strains with the Ighl-b allele. (C) 1998 Elsevier Science B.V. All rights reserved.
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- PROTEIN-A-SEPHAROSE; MOUSE SERUM; T-CELLS; GAMMA; GENES
- Publisher's Version
- https://doi.org/10.1016/S0022-1759(98)00015-5
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1998-03-15 12:00:00