A linkage between dendritic cell and T-cell development in the mouse thymus: The capacity of sequential T-cell precursors to form dendritic cells in culture

Lucas, K; Vremec, D; Wu, L; Shortman, K

Author(s): Lucas, K; Vremec, D; Wu, L; Shortman, K;
Details: Publication Year 1998-05,Volume 22,Issue #3,Page 339-349
Journal Title: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Publication Type: Journal Article
Abstract: The earliest T precursor population in the adult mouse thymus (CD4(lo)8(-)3(-)44(+)25(-) c-kit(+)) was previously shown to be lymphoid-restricted (T, B, NK) but to have a capacity to form dendritic cells (DC), This led to the concept of a lineage of lymphoid-derived DC, DC could be generated with high efficiency in culture from this low CD4 precursor, using a complex mix of cytokines, a mix that notably did not include GM-CSF, the cytokine normally used for development in culture of myeloid-derived DC. Using this new culture system we now show that the capacity to form DC extends to the pro-T precursor population (CD4(-)8(-)3(-) 44(+)25(+) c-kit(+)) but is lost by the pre-T precursor stage (CD4(-)8(-)3(-)44(-)25(+) c-kit(-)), the point of T-cell antigen-receptor beta-gene rearrangement, The DC generated in the cultures resemble mature thymic DC by most markers, but differ in their lack of expression of BP-1 and CD8 alpha. (C) 1998 Elsevier Science Ltd. All rights reserved.
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Keywords: CD8 EXPRESSION; STEM-CELLS; DIFFERENTIATION; COMMITMENT; PHENOTYPE; LINEAGE; PROGENITORS; KINETICS; ALPHA
Publisher's Version: https://doi.org/10.1016/S0145-305X(98)00012-3
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1998-05-01 12:00:00