Human phase I vaccine trials of 3 recombinant asexual stage malaria antigens with Montanide ISA720 adjuvant

Saul, A; Lawrence, G; Smillie, A; Rzepczyk, CM; Reed, C; Taylor, D; Anderson, K; Stowers, A; Kemp, R; Allworth, A; Anders, RF; Brown, GV; Pye, D; Schoofs, P; Irving, DO; Dyer, SL; Woodrow, GC; Briggs, WRS; Reber, R; STURCHLER, D

Author(s): Saul, A; Lawrence, G; Smillie, A; Rzepczyk, CM; Reed, C; Taylor, D; Anderson, K; Stowers, A; Kemp, R; Allworth, A; Anders, RF; Brown, GV; Pye, D; Schoofs, P; Irving, DO; Dyer, SL; Woodrow, GC; Briggs, WRS; Reber, R; STURCHLER, D;
Details: Publication Year 1999-08-06,Volume 17,Issue #23-24,Page 3145-3159
Journal Title: VACCINE
Publication Type: Journal Article
Abstract: Two phase I vaccine trials were conducted to test the immunogenicity and safety of a vaccine containing three recombinant malaria antigens from the asexual stage of Plasmodium falciparum. The three antigens are a fragment of MSPI (190LCS.T3); MSP2 and a portion of RESA and were formulated in Montanide ISA720 adjuvant, These trials investigated the dose response of each antigen for eliciting both antibody and T-cell responses and the immunogenicity of a mixture of the antigens compared with the antigens injected separately. All three antigens elicited both antibody and T-cell responses. Strong T-cell responses were observed with 190LCS,T3 and RESA with stimulation indices exceeding 100 for peripheral blood leucocytes in some individuals. The antibody responses were generally weak. The human antibody responses observed with MSP2 in Montanide ISA720 were nor significantly different from those obtained in an earlier trial which used MSP2 with alum as the adjuvant, No antigenic competition was observed: volunteers receiving a mixture of antigens had similar responses to those receiving the three antigens at separate sites. Tenderness and pain at the injection site were common over the first few days following immunization. In some volunteers, especially those receiving the highest doses tested, there was a delayed reaction at the injection site with pain and swelling occurring approximately 10 days after injection. (C) 1999 Elsevier Science Ltd. All rights reserved.
Publisher: ELSEVIER SCI LTD
Keywords: MEROZOITE SURFACE-ANTIGEN; PLASMODIUM-FALCIPARUM ANTIGEN; MONOCLONAL-ANTIBODY RECOGNIZES; NEW-GUINEAN CHILDREN; AOTUS MONKEYS; TERMINAL FRAGMENT; CLINICAL MALARIA; CHABAUDI-ADAMI; PROTEIN; IMMUNIZATION
Publisher's Version: https://doi.org/10.1016/S0264-410X(99)00175-9
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1999-08-06 12:00:00