Inhibition of naive class I-restricted T cells by altered peptide ligands
- Author(s)
- Koniaras, C; Carbone, FR; Heath, WR; Lew, AM;
- Details
- Publication Year 1999-08,Volume 77,Issue #4,Page 318-323
- Journal Title
- IMMUNOLOGY AND CELL BIOLOGY
- Publication Type
- Journal Article
- Abstract
- Amino acid variants of an antigenic peptide or altered peptide ligands have previously been investigated with CD4(+) and CD8(+) T cells. However, for CD8(+) T cells, only clones (which are continually restimulated in vitro) have been assessed. Using TCR transgenic mice specific for a class I Kb-restricted OVA peptide (OVAp; OT-I mice) as a source of naive CD8(+) T cells, single amino acid variants of the OVAp were analysed in vitro for their ability to antagonize the proliferative and cytotoxic function of naive OT-I cells. Peptides with substitutions at TCR contact residues were found to be the most potent antagonists of OT-I cell function. Those peptides that inhibited activation of cells to proliferate also inhibited activation of cells to become killers. Inhibition was inversely correlated with interferon (IFN)-gamma production. It was found that levels of antagonist peptide required for inhibition were higher than that described for T cell clones, presumably due to affinity differences.
- Publisher
- BLACKWELL SCIENCE ASIA
- Keywords
- EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; CYTOTOXIC LYMPHOCYTES-T; RECEPTOR ANTAGONISTS; OVALBUMIN PEPTIDE; INTERFERON-GAMMA; ANTIGEN; COMPLEX; TCR; EPITOPES; MHC
- Publisher's Version
- https://doi.org/10.1046/j.1440-1711.1999.00828.x
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1999-08-01 12:00:00