Molecular cloning and characterization of a novel repeat-containing Leishmania major gene, ppg1, that encodes a membrane-associated form of proteophosphoglycan with a putative glycosylphosphatidylinositol anchor
- Author(s)
- Ilg, T; Montgomery, J; Stierhof, YD; Handman, E;
- Details
- Publication Year 1999-10-29,Volume 274,Issue #44,Page 31410-31420
- Journal Title
- JOURNAL OF BIOLOGICAL CHEMISTRY
- Publication Type
- Journal Article
- Abstract
- Leishmania parasites secrete a variety of proteins that are modified by phosphoglycan chains structurally similar to those of the cell surface glycolipid lipophosphoglycan. These proteins are collectively called proteophosphoglycans. We report here the cloning and sequencing of a novel Leishmania major proteophosphoglycan gene, ppg1. It encodes a large polypeptide of approximately 2300 amino acids. The N-terminal domain of approximately 70 kDa exhibits 11 imperfect amino acid repeats that show some homology to promastigote surface glycoproteins of the psa2/gp46 complex. The large central domain apparently consists exclusively of approximately 100 repetitive peptides of the sequence APSASSSSA(P/S)SSSSS(+/-S). Gene fusion experiments demonstrate that these peptide repeats are the targets of phosphoglycosylation in Leishmania and that they form extended filamentous structures reminiscent of mammalian mucins. The C-terminal domain contains a functional glycosylphosphatidylinositol anchor addition signal sequence, which confers cell surface localization to a normally secreted Leishmania acid phosphatase when fused to its C terminus. Antibody binding studies show that the ppg1 gene product is phosphoglycosylated by phosphoglycan repeats and cap oligosaccharides. In contrast to previously characterized proteophosphoglycans, the ppg1 gene product is predominantly membrane-associated and it is expressed on the promastigote cell surface. Therefore this membrane-bound proteophosphoglycan may be important for direct host-parasite interactions.
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Keywords
- SECRETED ACID-PHOSPHATASE; SURFACE-ANTIGEN; GLYCOSYLATED PHOSPHATIDYLINOSITOLS; STRUCTURAL CHARACTERIZATION; LINKED OLIGOSACCHARIDES; MONOCLONAL-ANTIBODIES; MEXICANA AMASTIGOTES; TRYPANOSOMA-BRUCEI; LIPOPHOSPHOGLYCAN; PROMASTIGOTES
- Publisher's Version
- https://doi.org/10.1074/jbc.274.44.31410
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- Refer to copyright notice on published article.
Creation Date: 1999-10-29 12:00:00