The proinflammatory and chondral activities of leukemia inhibitory factor in goat joints are partially a function of interleukin-1
- Author(s)
- Bell, MC; Carroll, GJ; Chapman, HM; Mills, JN; Layton, MJ; Saklatvala, J;
- Details
- Publication Year 1999-02,Volume 19,Issue #2,Page 197-208
- Journal Title
- JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
- Publication Type
- Journal Article
- Abstract
- We wished to determine if the effects of injected recombinant human leukemia inhibitory factor (LIF) are a function of endogenous goat interleukin-1 (IL-1) production and, conversely, if the effects of injected recombinant human IL-1 are a function of endogenous LIF production in goat radiocarpal joints (RCJ). In preliminary experiments, murine LIF binding protein (MuLBP) and recombinant HuIL-1RA were found to independently attenuate the cartilage proteoglycan resorbing activity of goat synovial membrane-conditioned medium (GSMCM), implying activity against goat LIF and goat IL-1, respectively. The present study shows that the proinflammatory and chondral actions of rHuLIF in goat RCJ are partially attenuated by rHuIL-1RA. This implies that a small but important component of the in vivo activity of rHuLIF is a result of IL-1 production in the synovial joint. With the exception of proteoglycan synthesis, the absence of significant effects by MuLBP on the actions of rHuIL-1 alpha in goat RCJ suggests that the proinflammatory and chondral effects of IL-1 alpha in vivo are probably not mediated by LIF.
- Publisher
- MARY ANN LIEBERT INC PUBL
- Keywords
- CARTILAGE PROTEOGLYCAN DEGRADATION; NECROSIS-FACTOR-ALPHA; ARTICULAR-CARTILAGE; FACTOR LIF; RHEUMATOID-ARTHRITIS; LEUKOCYTE INFILTRATION; RECEPTOR ANTAGONIST; EMBRYONAL CARCINOMA; CONDITIONED MEDIUM; BINDING-PROTEIN
- Publisher's Version
- https://doi.org/10.1089/107999099314342
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1999-02-01 12:00:00