Differential mRNA display analysis of two related but functionally distinct rat insulinoma (RIN) cell lines: identification of CD24 and its expression in the developing pancreas
- Author(s)
- Cram, DS; McIntosh, A; Oxbrow, L; Johnston, AM; DeAizpurua, HJ;
- Details
- Publication Year 1999-05,Volume 64,Issue #4,Page 237-246
- Journal Title
- DIFFERENTIATION
- Publication Type
- Journal Article
- Abstract
- To identify genes that might play a role in growth and differentiation of pancreatic beta-cells, we have applied the technique of differential mRNA display to the lineage-related, but functionally distinct rat insulinoma (RIN) cell lines RIN-5AH and RIN-A12. Direct comparison of PCR-generated RIN-5AH and RIN-A12 cDNAs on DNA sequencing gels revealed 31 differentially expressed bands. By Northern blot hybridization, authentic differential expression was confirmed for three cDNAs derived from RIN-SAH cells and four cDNAs from RIN-A12 cells. Nucleotide sequences were determined for these cDNAs and database searches identified one known gene that encoded heat stable antigen CD24. Of the remaining six genes, three matched with established sequence tags from fetal tissue, and three were potentially novel. By RT-PCR analysis, five of the seven genes were expressed in normal fetal and/or adult pancreas. In a detailed survey of CD24 protein expression in the pancreas using the CD24-specific monoclonal antibody J11d, CD24 was predominantly expressed in ductal epithelial cells (E13.5-15.5), developing endocrine (alpha, beta and delta) and exocrine cells (E15.5-20.5) and mature exocrine and peripheral islet delta-cells post E20.5. The retention of CD24 expression in a large proportion of delta-cells but only in a minority of alpha- and beta-cells leads us to hypothesize that CD24 may mark a pool of precursor endocrine cells within adult islets.
- Publisher
- SPRINGER VERLAG
- Keywords
- HEAT-STABLE ANTIGEN; A2B5-REACTIVE GANGLIOSIDE EXPRESSION; ISLET CELLS; SIGNAL-TRANSDUCTION; GENE-EXPRESSION; BETA-CELLS; MOUSE CD24; P-SELECTIN; MOLECULE; PEPTIDE
- Publisher's Version
- https://doi.org/10.1046/j.1432-0436.1999.6440237.x
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- Refer to copyright notice on published article.
Creation Date: 1999-05-01 12:00:00