Pax-3 regulates neurogenesis in neural crest-derived precursor cells
- Author(s)
- Koblar, SA; Murphy, M; Barrett, GL; Underhill, A; Gros, P; Bartlett, PF;
- Details
- Publication Year 1999-06-01,Volume 56,Issue #5,Page 518-530
- Journal Title
- JOURNAL OF NEUROSCIENCE RESEARCH
- Publication Type
- Journal Article
- Abstract
- The peripheral nervous system consists of multiple neural lineages derived from the neural crest (NC), Pax-3 is expressed in the NC and when mutated in the splotch mouse (Sp) results in the loss of derivatives from this precursor cell population, We have investigated the role of Pax-3 in regulating the generation of neurons from NC-derived precursor cells in vitro. Pax-3 mRNA in NC cultures is initially expressed in all NC but is subsequently only retained in neurons, suggesting a role in their generation. To determine whether Pax-3 is involved in neuron development, we first examined the generation of sensory-like neurons in NC cultures from Sp mice. Fivefold less sensory-like neurons were generated in NC cultures from Sp homozygous mice as compared to wild-type littermates. The role of Pax-3 in sensory neuron generation was then directly examined in dorsal root ganglia cultures by down-regulating the expression of Pax-3 protein with antisense oligonulceotides, It was found that antisense oligonulceotides inhibited 80-90% of newly generated sensory neurons; however, there was no significant effect on the survival of sensory neurons or the precursor population. These results suggest that Pax-3 has a role in regulating the differentiation of peripheral neurons. (C) 1999 Wiley-Liss,Inc.
- Publisher
- WILEY-LISS
- Keywords
- LEUKEMIA INHIBITORY FACTOR; CENTRAL-NERVOUS-SYSTEM; SPLOTCH MUTANT MOUSE; GROWTH-FACTOR; VERTEBRATE DEVELOPMENT; MUSCLE DEVELOPMENT; PAIRED DOMAIN; STEM-CELLS; GENE; EXPRESSION
- Publisher's Version
- https://doi.org/10.1002/(SICI)1097-4547(19990601)56:5<518::AID-JNR7>3.0.CO;2-8
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- Refer to copyright notice on published article.
Creation Date: 1999-06-01 12:00:00