CLONING OF A MURINE IL-11 RECEPTOR ALPHA-CHAIN - REQUIREMENT FOR GP130 FOR HIGH-AFFINITY BINDING AND SIGNAL-TRANSDUCTION
- Author(s)
- Hilton, DJ; Hilton, AA; RAICEVIC, A; Rakar, S; HarrisonSmith, M; Gough, NM; Begley, CG; Metcalf, D; Nicola, NA; Willson, TA;
- Details
- Publication Year 1994-10-17,Volume 13,Issue #20,Page 4765-4775
- Journal Title
- EMBO JOURNAL
- Publication Type
- Journal Article
- Abstract
- An adult mouse liver cDNA library was screened with oligonucleotides corresponding to the conserved WSXWS motif of the haemopoietin receptor family. Using this method, cDNA clones encoding a novel receptor were isolated. The new receptor, named NR1, was most similar in sequence and predicted structure to the a-chain of the IL-6 receptor and mRNA was expressed in the 3T3-L1 pre-adipocytic cell line and in a range of primary tissues. Expression of NR1 in the factor-dependent haemopoietic cell line Ba/F3 resulted in the generation of low affinity receptors for IL-11 (K-d approximate to 10 nM). The capacity to bind IL-11 with high affinity (K-d = 300-800 pM) appeared to require coexpression of both NR1 and gp130, the common subunit of the IL-6, leukaemia inhibitory factor (LIF), oncostatin M (OSM) and ciliary neurotrophic factor (CNTF) receptors. The expression of both NR1 and gp130 was also necessary for Ba/F3 cells to proliferate and M1 cells to undergo macrophage differentiation in response to IL-11.
- Publisher
- OXFORD UNIV PRESS UNITED KINGDOM
- Keywords
- LEUKEMIA-INHIBITORY FACTOR; TUMOR-NECROSIS-FACTOR; COLONY-STIMULATING FACTOR; MOLECULAR-CLONING; ONCOSTATIN-M; BIOLOGICAL CHARACTERIZATION; HEMATOPOIETIC PROGENITORS; SYNERGISTIC INTERACTIONS; SURFACE-ANTIGEN; TYROSINE KINASE
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Creation Date: 1994-10-17 12:00:00