MOLECULAR-CLONING OF A GENE ENCODING AN ARABINOGALACTAN PROTEIN FROM PEAR (PYRUS-COMMUNIS) CELL-SUSPENSION CULTURE
Details
Publication Year 1994-10-25, Volume 91, Issue #22, Page 10305-10309
Journal Title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publication Type
Journal Article
Abstract
Arabinogalactan proteins (AGPs) are proteoglycans containing a high proportion of carbohydrate (typically >90%) linked to a protein backbone rich in hydroxyproline (Hyp), Ala, Ser, and Thr. They are widely distributed in plants and may play a role in development. The structure of the carbohydrate of some AGPs is known in detail but information regarding the protein backbone is restricted to a few peptide sequences. Here we report isolation and partial amino acid sequencing of the protein backbone of an AGP, This AGP is a member of one of four major groups of AGPs isolated from the filtrate of pear cell suspension culture. A cDNA encoding this protein backbone (145 amino acids) was cloned; the deduced protein is rich in Hyp, Ala, Ser, and Thr, which together account for >75% of total residues. It has three domains, an N-terminal secretion signal, a central hydrophilic domain containing all of the Pro residues, and a hydrophobic C-terminal domain that is predicted to be a transmembrane helix. Approximately 93% of the Pro residues are hydroxylated and hence are potential sites for glycosylation.
Publisher
NATL ACAD SCIENCES
Keywords
PLASMA-MEMBRANE; DAUCUS-CAROTA; SEQUENCE; CHROMATOGRAPHY; PURIFICATION
Rights Notice
Refer to copyright notice on published article.


Creation Date: 1994-10-25 12:00:00
Last Modified: 0001-01-01 12:00:00
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