SULFATED GLYCOCONJUGATES AS DISRUPTERS OF PLASMODIUM-FALCIPARUM ERYTHROCYTE ROSETTES
- Author(s)
- Rogerson, SJ; Reeder, JC; AlYaman, F; Brown, GV;
- Details
- Publication Year 1994-08,Volume 51,Issue #2,Page 198-203
- Journal Title
- AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
- Publication Type
- Journal Article
- Abstract
- Some strains of Plasmodium falciparum form erythrocyte rosettes that are believed to result from a lectin interaction between malaria-infected and uninfected erythrocytes. The sulfated glycoconjugate heparin and certain heparin derivatives have been observed to disrupt rosettes. To investigate this interaction further, we have studied the effects of four sulfated glycoconjugates on 15 fresh isolates of P. falciparum from Papua New Guinea. A broader range of sulfated glycoconjugates has been tested against a laboratory strain. A concentration of 1,000 mu g/ml of dextran sulfate (molecular weight [MW] 500,000) was the most potent disrupter of rosettes. Fucoidan, heparin, and dextran sulfate (MW 5,000) were of decreasing effectiveness in 14 of 15 fresh isolates. The same relationship was true for the laboratory strain. Pentosan polysulfate and sulfatide also disrupted rosettes; chondroitin sulfates A, B, and C and keratan sulfate gave either minimal or no rosette disruption. Thus, some sulfated glycoconjugates are potent disrupters of P. falciparum erythrocyte rosettes. Sulfated glycoconjugaes that are potent disrupters of P. falciparum rosettes may prove useful in identifying ligands involved in rosette formation.
- Publisher
- AMER SOC TROP MED & HYGIENE
- Keywords
- MALARIAL CIRCUMSPOROZOITE PROTEIN; INFECTED ERYTHROCYTES; CONTINUOUS CULTURE; SEQUENCE HOMOLOGY; HEPARAN-SULFATE; REGION-II; BINDS; CYTOADHERENCE; SPOROZOITES; GLYCOLIPIDS
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Creation Date: 1994-08-01 12:00:00