CHARACTERIZATION OF A POLYMORPHIC FAMILY OF INTEGRAL MEMBRANE-PROTEINS IN PROMASTIGOTES OF DIFFERENT LEISHMANIA SPECIES
- Author(s)
- SYMONS, FM; Murray, PJ; Ji, H; Simpson, RJ; Osborn, AH; Cappai, R; Handman, E;
- Details
- Publication Year 1994-09,Volume 67,Issue #1,Page 103-113
- Journal Title
- MOLECULAR AND BIOCHEMICAL PARASITOLOGY
- Publication Type
- Journal Article
- Abstract
- Antibodies raised against a Leishmania major recombinant promastigote surface antigen 2 (PSA-2) fragment recognized three major polypeptides of approximate M(r) 96 000, 80 000 and 50 000 in promastigotes of three Israeli isolates of L. major including the cloned line LRC-L137-V121, but detected a different array of polypeptides in other L. major isolates. The pattern was different both in number of polypeptides detected and their molecular weight. The antibodies to L. major PSA-2 also recognized polypeptides in L. tropica, L. donovani and very weakly in L. mexicana promastigotes and in Crithidia lucilliae. The number and size of the polypeptides was different in each species. In addition to the membrane-bound PSA-2 polypeptides were identified water-soluble forms of PSA-2 released in promastigote culture supernatants. Peptide maps of the various L. major PSA-2 membrane polypeptides showed they were different from each other. N-terminal amino acid sequence of three polypeptides expressed by L. major showed they are similar but distinct, consistent with being members of a polymorphic family. Because of the extensive sequence similarity between the PSA-2 genes it has been difficult to assign protein products to individual genes. As a first step towards solving this problem, we have transfected into L. mexicana a genomic clone of a L. major PSA-2 gene and shown that it produces a M(r) 35 000 polypeptide recognized by monoclonal and polyclonal antibodies to L. major PSA-2.
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- SODIUM DODECYL-SULFATE; SURFACE-ANTIGEN; MONOCLONAL-ANTIBODIES; POLYACRYLAMIDE GELS; GLYCOPROTEIN; MACROPHAGES; AMAZONENSIS; EXPRESSION; VIRULENCE; INFECTION
- Publisher's Version
- https://doi.org/10.1016/0166-6851(94)90100-7
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1994-09-01 12:00:00