ISOLATION AND CHARACTERIZATION OF GROUP-I ISOALLERGENS FROM BERMUDA GRASS-POLLEN
- Author(s)
- SMITH, PM; AVJIOGLU, A; WARD, LR; Simpson, RJ; KNOX, RB; Singh, MB;
- Details
- Publication Year 1994-05,Volume 104,Issue #1,Page 57-64
- Journal Title
- INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Two isoallergens of Cyn d I were isolated using preparative isoelectric focussing, and were designated Cyn d Ia and b. These isoallergens differ in their pI, molecular weight (Cyn d Ia, 32 kD and Cyn d Ib, 31 kD) and their NH2-terminal sequence. Four monoclonal antibodies (Mabs) were produced using Cyn d Ia as antigen. These Mabs recognized both Cyn d Ia and b. One of the Pollen Allergen Mabs recognized four different pI forms of Cyn d I on 2D gels. The Mabs also Bermuda grass recognized cross-reactive epitopes on proteins from eight other grasses including rye grass, timothy grass, Kentucky bluegrass and Johnson grass.
- Publisher
- KARGER
- Keywords
- MAJOR ALLERGENIC PROTEIN; CYNODON-DACTYLON POLLEN; LOLIUM-PERENNE POLLEN; MONOCLONAL-ANTIBODIES; P-I; CROSS-ALLERGENICITY; POLYACRYLAMIDE GELS; IGE ANTIBODIES; V-ALLERGENS; IDENTIFICATION
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Creation Date: 1994-05-01 12:00:00