RECEPTOR INSERTION INTO FACTOR-DEPENDENT MURINE CELL-LINES TO DEVELOP SPECIFIC BIOASSAYS FOR MURINE G-CSF AND M-CSF AND HUMAN GM-CSF
- Author(s)
- Metcalf, D; Willson, T; ROSSNER, M; Lock, P;
- Details
- Publication Year 1994,Volume 11,Issue #2,Page 145-152
- Journal Title
- GROWTH FACTORS
- Publication Type
- Journal Article
- Abstract
- cDNAs encoding the receptors for murine G-CSF, M-CSF or human GM-CSF were inserted into the murine hemopoietic continuous cell lines Ba/F3 or FDC-P1 and sublines selected that were then responsive to proliferative stimulation by these growth factors. When used in microwell assays the Ba/F3 G-CSF receptor-expressing cell line was able to detect 100 pg G-CSF per mi, the Ba/F3 M-CSF receptor-expressing cell line 100-400 pg M-CSF per mi and the EDC-P1 line expressing the alpha- and beta-chains of the human GM-CSF receptor detected 5-10 pg/ml of GM-CSF in test material. These cell lines appear satisfactory for use as selective bioassays for these colony stimulating factors in material potentially containing a mixture of growth factors.
- Publisher
- HARWOOD ACAD PUBL GMBH
- Keywords
- COLONY-STIMULATING FACTOR; GROWTH
- Publisher's Version
- https://doi.org/10.3109/08977199409001056
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1994-01-01 12:00:00