ALLELIC VARIANTS OF THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE-ANTIGEN-2 (MSA-2) IN A GEOGRAPHICALLY RESTRICTED AREA OF IRIAN-JAYA
- Author(s)
- Marshall, VM; ANTHONY, RL; Bangs, MJ; PURNOMO; Anders, RF; Coppel, RL;
- Details
- Publication Year 1994-01,Volume 63,Issue #1,Page 13-21
- Journal Title
- MOLECULAR AND BIOCHEMICAL PARASITOLOGY
- Publication Type
- Journal Article
- Abstract
- Blood samples were collected from 12 residents of 4 villages in the Oksibil area of Irian Jaya. Eleven patients were positive for Plasmodium falciparum infection as evidenced by successful amplification of the MSA-2 gene by the polymerase chain reaction. Two patients showed evidence of infection by 2 strains of Plasmodium falciparum. All MSA-2 genes were completely sequenced and all could be assigned to one of the two major allelic families of MSA-2, however all MSA-2 gene sequences differed from previously described alleles. Five new allelic forms were identified, one of which was present in 8 of the 11 patients. Within small natural populations of P. falciparum, it appears that variation in MSA-2 approximates that seen world-wide. All samples were also analysed by hybridisation of amplified DNA to family specific probes and all samples hybridised to known probes. Our results demonstrate that there is a degree of microheterogeneity of MSA-2 that is undetectable by hybridisation studies alone.
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- STRUCTURAL DIVERSITY; GENETIC DIVERSITY; MALARIA; POLYMORPHISM
- Publisher's Version
- https://doi.org/10.1016/0166-6851(94)90004-3
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1994-01-01 12:00:00