A FORM OF IMMUNOLOGICAL-TOLERANCE THROUGH IMPAIRMENT OF GERMINAL CENTER DEVELOPMENT
- Author(s)
- Pulendran, B; KARVELAS, M; Nossal, GJV;
- Details
- Publication Year 1994-03-29,Volume 91,Issue #7,Page 2639-2643
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- Primary immunization with the T-cell-dependent antigen (4-hydroxy-3-nitrophenyl)acetyl (NP) coupled to human serum albumin results in the development of two pathways of B-cell development, the extrafollicular pathway and the germinal center pathway. Soluble, deaggregated NP-human serum albumin injected before immunization results in a marked diminution of clonable higher-affinity antibody-forming cell precursors-i.e., a form of immunologic tolerance within the secondary B-cell repertoire. We describe here the cellular changes in the spleen that underlie this tolerance. Using multiparameter flow cytometry, we show that tolerant mice develop far fewer NP-binding, peanut agglutinin-positive, or germinal center cells than the control immunized mice; 14 days after challenge control spleens have almost-equal-to 2 x 10(5) such cells per spleen, whereas the tolerant mice have almost-equal-to 1 x 10(4) cells. Furthermore, we demonstrate by immunohistology a redction in the number of germinal centers containing lambda-bearing cells, characteristic of the response of C57BL/6 mice to NP. Taken together, these data suggest an impairment of germinal center development in the tolerant mice.
- Publisher
- NATL ACAD SCIENCES
- Keywords
- IMMUNE-RESPONSE; C57BL-6 MICE; B-CELLS; MATURATION; (4-HYDROXY-3-NITROPHENYL)ACETYL; ANTIBODIES; NP
- Publisher's Version
- https://doi.org/10.1073/pnas.91.7.2639
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1994-03-29 12:00:00