A YAC CONTIG MAP OF PLASMODIUM-FALCIPARUM CHROMOSOME-4 - CHARACTERIZATION OF A DNA AMPLIFICATION BETWEEN 2 RECENTLY SEPARATED ISOLATES
- Author(s)
- Rubio, JP; Triglia, T; Kemp, DJ; DEBRUIN, D; Ravetch, JV; Cowman, AF;
- Details
- Publication Year 1995-03-20,Volume 26,Issue #2,Page 192-198
- Journal Title
- GENOMICS
- Publication Type
- Journal Article
- Abstract
- We have generated a physical map of Plasmodium falciparum chromosome 4 using yeast artificial chromosomes (YACs). The map is defined by a YAC contig spanning approximately 1.05 Mb, which has been restriction mapped to a resolution of 30 kb and is punctuated by 22 sequence tagged sites. The physical information obtained has enabled us to compare and contrast the structure of chromosome 4 in detail between FCR3 and B8, two recently separated isolates of P. falciparum, leading to characterization of a novel chromosome polymorphism occurring in a subtelomeric region. Comparison of chromosomes 4 from 10 different isolates has shown that chromosome size polymorphisms are restricted to both subtelomeric regions. These analyses provide a high-resolution physical map that will be important to complement genetic analysis of this human pathogen. (C) 1995 Academic Press, Inc.
- Publisher
- ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
- Keywords
- YEAST ARTIFICIAL CHROMOSOMES; THYMIDYLATE SYNTHASE GENE; MULTIDRUG RESISTANCE GENE; MALARIA PARASITE; P-FALCIPARUM; SEQUENCE-ANALYSIS; SIZE VARIATION; DELETIONS; FRAGMENTS; CLONING
- Publisher's Version
- https://doi.org/10.1016/0888-7543(95)80200-6
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1995-03-20 12:00:00