C-TERMINAL EXTENSION OF TRUNCATED RECOMBINANT PROTEINS IN ESCHERICHIA-COLI WITH A 10SA RNA DECAPEPTIDE
- Author(s)
- Tu, GF; Reid, GE; Zhang, JG; Moritz, RL; Simpson, RJ;
- Details
- Publication Year 1995-04-21,Volume 270,Issue #16,Page 9322-9326
- Journal Title
- JOURNAL OF BIOLOGICAL CHEMISTRY
- Publication Type
- Journal Article
- Abstract
- When murine interleukin-6 is overexpressed in Escherichia coli, a small population of molecules exhibits a novel C-terminal modification. Peptide mapping, electrospray ionization-mass spectrometry, and automated N- and C-terminal sequencing identified a peptide (''tag'' peptide), Ala-Ala-Asn-Asp-Glu-Asn-Tyr-Aza-Leu-Ala COOH, encoded by a small metabolically stable RNA of E. coli (10Sa RNA) attached to truncated C termini of the recombinant protein. A mutant strain of E. coli in which the chromosomal 10Sa RNA gene (ssrA) is disrupted does not produce this C-terminal modification, confirming that the tag peptide originates from the ssrA gene.
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Keywords
- SMALL STABLE RNA; MURINE INTERLEUKIN-6; SYNTHETIC GENE; PURIFICATION; EXPRESSION; SEQUENCE
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1995-04-21 12:00:00