ANTIENDOMETRIAL LYMPHOCYTOTOXICITY AND NATURAL-KILLER-CELL ACTIVITY IN BABOONS (PAPIO-ANUBIS AND PAPIO-CYNOCEPHALUS) WITH ENDOMETRIOSIS
- Author(s)
- DHOOGHE, TM; Scheerlinck, JPY; KONINCKX, PR; HILL, JA; BAMBRA, CS;
- Details
- Publication Year 1995-03,Volume 10,Issue #3,Page 558-562
- Journal Title
- HUMAN REPRODUCTION
- Publication Type
- Journal Article
- Abstract
- This study was performed to test the hypothesis that antiendometrial, lymphocyte-mediated cytotoxicity and natural killer (NK) activity are reduced in baboons with endometriosis when compared to animals with a normal pelvis, Lymphocyte-mediated cytotoxicity was determined in 28 baboons (15 with endometriosis, 13 with normal pelvis) and NK cell activity was evaluated in 42 baboons (31 with endometriosis, 11 with normal pelvis), Anti-endometrial lymphocyte-mediated cytotoxicity was determined by a 20 h assay with effector-target ratios of 50:1 and 25:1. The NK activity (K562 cell line as target) was simultaneously measured in all animals during a 4 h assay with effector:target ratios of 200:1, 100:1, 50:1, 25:1, 12:1, 6:1 and 3:1, Statistical analysis was performed using analysis of variance, paired rank, Mann-Whitney, Kruskal-Wallis and Fisher exact tests where appropriate, Lymphocyte-mediated cytotoxicity was significantly lower (P < 0.025) in baboons with endometriosis (mean 5.9 +/- 8.7%, median 0%, range 0-26%) than in animals with a normal pelvis (mean 22.9 +/- 23.0%, median 7%, range 0-78%), This difference could be explained by the absence of cytotoxicity in baboons with moderate to severe endometriosis, probably due to high spontaneous release of Cr-51 from labelled target cells. When stricter criteria were used and only animals with a labelling index (maximal/spontaneous release) of greater than or equal to 1.7 were analysed (n = 11), the anti-endometrial cytotoxicity was comparable between baboons with and without endometriosis. NK cell activity was also comparable in primates with and without endometriosis. In conclusion, no difference in lymphocyte-mediated cytotoxicity and NK cell activity was observed between baboons with and without endometriosis.
- Publisher
- OXFORD UNIV PRESS UNITED KINGDOM
- Keywords
- RETROGRADE MENSTRUATION; WOMEN; CYTOTOXICITY
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1995-03-01 12:00:00