CHARACTERIZATION OF THE GENE FAMILY ENCODING A HOST-PROTECTIVE ANTIGEN OF THE TAPEWORM TAENIA-OVIS
- Author(s)
- Waterkeyn, JG; Lightowlers, MW; Coppel, R; Cowman, AF;
- Details
- Publication Year 1995-07,Volume 73,Issue #1-2,Page 123-131
- Journal Title
- MOLECULAR AND BIOCHEMICAL PARASITOLOGY
- Publication Type
- Journal Article
- Abstract
- Genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth Taenia ovis. An incomplete cDNA, known as 45W, encodes the protective antigen. Southern hybridisation experiments using 45W cDNA as a probe, revealed that the 45W gene was a member of a multigene family. Differential Southern hybridisation and rapid amplification of cDNA end (RACE) experiments were used to characterise the related genes, allowing the full-length coding region of the 45W encoded antigen to be determined. The gene family comprises a minimum of four members per haploid genome with each member showing varying degrees of 5' and 3' homology with respect to the 45W cDNA. A close homologue of the 45W gene, designated 45S, differed from 45W at 11 of 985 nt comprising the full-length mRNA. Sequencing of several independent RACE products for both 45W and 45S identified a cDNA which may be a product of homologous recombination between these genes, suggesting that the two genes may be alleles. Homologous recombination in genes which encode a host protective antigen such as 45W would provide a mechanism by which antigenic variants could arise.
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- EUKARYOTIC MESSENGER-RNA; SEQUENCES; ECHINOCOCCUS; CESTODA; CLONING; SITE
- Publisher's Version
- https://doi.org/10.1016/0166-6851(94)00104-U
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1995-07-01 12:00:00