IDENTIFICATION OF CD36 AS THE FIRST GENE DEPENDENT ON THE B-CELL DIFFERENTIATION FACTOR OCT-2

KONIG, H; PFISTERER, P; Corcoran, LM; WIRTH, T

Author(s): KONIG, H; PFISTERER, P; Corcoran, LM; WIRTH, T;
Details: Publication Year 1995-07-01,Volume 9,Issue #13,Page 1598-1607
Journal Title: GENES & DEVELOPMENT
Publication Type: Journal Article
Abstract: The Oct-2 transcription factor is expressed predominantly in B lymphocytes and has been shown previously to be important for the terminal phase of B-cell differentiation in mice. A number of genes specifically expressed in B cells contain Oct-2-binding sites in their regulatory regions. However, the analysis of expression levels of these genes in Oct-2-deficient B cells revealed that they were unaffected. Hence, there were no genes known that critically depend on Oct-2 for their expression. To understand the molecular basis for the Oct-2 effect on B-cell development, we searched for Oct-2 target genes by subtractive cDNA cloning. We show here that expression of the murine CD36 gene in B cells and macrophages requires a functional Oct-2 protein. Nuclear run-on experiments demonstrate that this gene is regulated transcriptionally by Oct-2. Moreover, CD36 levels correlated with the levels of Oct-2 expression in several mouse B-cell and macrophage cell lines. finally, compared to wild-type and heterozygous mice, CD36 mRNA levels were markedly reduced in spleens and B cell-enriched splenocyte fractions from oct-2(-/-) mice. The data identify CD36 as the first target gene critically dependent on Oct-2 for its expression. Because CD36 expression is also dependent on Oct-2 in vivo, it is a candidate gene through which Oct-2 could affect B-cell differentiation.
Publisher: COLD SPRING HARBOR LAB PRESS
Keywords: FOLLICULAR DENDRITIC CELLS; GLYCOPROTEIN-IV CD36; CHAIN FATTY-ACIDS; OCTAMER DNA MOTIF; MEMBRANE GLYCOPROTEIN; IMMUNOGLOBULIN PROMOTERS; TRANSCRIPTION FACTORS; PROTEIN-BINDING; SEQUENCE MOTIF; BOVINE-MILK
Publisher's Version: https://doi.org/10.1101/gad.9.13.1598
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1995-07-01 12:00:00