HIV-1 INFECTION OF HUMAN MACROPHAGES IMPAIRS PHAGOCYTOSIS AND KILLING OF TOXOPLASMA-GONDII
- Details
- Publication Year 1995-06-01,Volume 154,Issue #11,Page 6132-6139
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- The susceptibility of patients with AIDS to certain opportunistic infections is due to defective cell-mediated immunity. The contribution of direct infection of macrophages with HIV-1 to this defect is unknown. To address this issue, we infected normal human monocyte-derived macrophages with a monocytotropic strain of HIV-1 and examined their ability to phagocytose and kill the opportunistic pathogen, Toxoplasma gondii. Phagocytosis of heat-killed T. gondii was reduced in HIV-infected macrophages compared with mock-infected controls, Opsonization of heal-killed T. gondii increased phagocytosis by both mock- and HIV-infected macrophages, but phagocytosis in HIV-infected cultures remained lower than in controls. Internalization of live T. gondii by macrophages was unaffected by HIV infection. Intracellular replication of live T. gondii was enhanced by HIV infection, as shown in four experiments, each using monocyte-derived macrophages from a different donor. Treatment of HIV-infected macrophages with IFN-gamma decreased parasite replication but not to control levels. These findings suggest that infection of macrophages by HIV may be a contributing factor to the reactivation of T.gondii infection in patients with AIDS.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- HUMAN-IMMUNODEFICIENCY-VIRUS; MOUSE PERITONEAL-MACROPHAGES; INTERFERON-GAMMA; MYCOBACTERIUM-AVIUM; AIDS PATIENTS; MONONUCLEAR PHAGOCYTES; ACTIVATED MACROPHAGES; MONOCYTE FUNCTION; HOST-CELLS; EXPRESSION
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1995-06-01 12:00:00