INTERFERON-GAMMA DOWN-REGULATES THE PROLIFERATIVE RESPONSE OF HAPTEN-SPECIFIC B-CELLS STIMULATED BY ANTIGEN AND CYTOKINES
- Details
- Publication Year 1995-02,Volume 73,Issue #1,Page 52-56
- Journal Title
- IMMUNOLOGY AND CELL BIOLOGY
- Publication Type
- Journal Article
- Abstract
- IFN-gamma plays a role in many aspects of cellular interactions, both positive and negative. Among its functions during the immune response, the antagonistic effects of IFN-gamma and IL-4 are well documented. Observations in our laboratory suggested that IFN-gamma could also interfere with the activation of single, antigen-specific B cells by antigen and other cytokines. Closer examination revealed that IFN-gamma reduced the number of proliferating cell clones in response to antigen and a variety of cytokines, alone or in combination. Cell viability remained at the initial level and the cells were still able to produce Ig, albeit to a lesser extent than in the absence of IFN-gamma. On the other hand, the frequency of IgM secreting clones was not affected, whereas the total amount of secreted IgM was lower in the presence of IFN-gamma, probably due to the reduced cell number and a decrease in Ig production. In addition, proliferation was prevented when B cells were pre-incubated with IFN-gamma and then stimulated by other cytokines. Kinetic studies revealed that INF-gamma had to be present from the onset of culture because delayed addition did not inhibit the proliferation of the B cells. After its initial action, IFN-gamma could be removed without abolishing the negative signal for proliferation. From these results it can be concluded that IFN-gamma transmits a signal that causes B cells to stop proliferating and prevents them from forming large clones.
- Publisher
- BLACKWELL SCIENCE PUBL AUSTR
- Keywords
- LYMPHOCYTES; ANTIBODY; SINGLE; LYMPHOKINES; ACTIVATION; GROWTH
- Publisher's Version
- https://doi.org/10.1038/icb.1995.8
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1995-02-01 12:00:00