THE SUBUNIT COMPOSITION OF NF-KAPPA-B COMPLEXES CHANGES DURING B-CELL DEVELOPMENT
- Author(s)
- Grumont, RJ; Gerondakis, S;
- Details
- Publication Year 1994-12,Volume 5,Issue #12,Page 1321-1331
- Journal Title
- CELL GROWTH & DIFFERENTIATION
- Publication Type
- Journal Article
- Abstract
- The transcription factor NF-kappa B, shown to be essential for expression of the immunoglobulin C kappa gene, is a key regulatory component in pre-B to B-cell differentiation. While previous studies have used lymphoid cell line models, here we examine the expression and subunit composition of rel/NF-kappa B complexes in normal murine pre-B and B lymphocytes. Two major NF-kappa B complexes are detected in pre-B and B cells. A high mobility complex, found in pre-B (C-b) and B cells (C beta) is a homodimer of the NF-kappa B subunit p50. In pre-B cells, the slower migrating complex (Ca), which is predominantly cytoplasmic, is largely comprised of p50 and p65, whereas in B cells, a nuclear and cytoplasmic complex (C alpha) of identical mobility to C-a mainly consists of p50 and p75(c-rel). While p50 and p65 levels do not change during pre-B to B-cell differentiation, p75(c-rel) is 5- to 6-fold more abundant in B cells compared to pre-B cells, a finding consistent with the switch of NF-kappa B subunit usage. During lipopolysaccharide-induced B-cell proliferation, transient up-regulation of both the nuclear p50 homodimer and p75(c-rel) containing complex is mirrored by a concurrent increase in c-rel and p105 but not p65 mRNA expression, a finding consistent with rel-NF-kappa B expression in B cells being controlled by an autoregulatory mechanism.
- Publisher
- AMER ASSOC CANCER RESEARCH
- Keywords
- DNA-BINDING SUBUNIT; C-REL TRANSCRIPTION; MONOCLONAL-ANTIBODY; MESSENGER-RNAS; PHORBOL ESTER; LYMPHOCYTE-B; T-CELLS; GENE; PROTEIN; ONCOGENE
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Creation Date: 1994-12-01 12:00:00