RECONSTITUTION OF MICE WITH BONE-MARROW CELLS EXPRESSING THE SCL GENE IS INSUFFICIENT TO CAUSE LEUKEMIA
- Author(s)
- Elwood, NJ; Begley, CG;
- Details
- Publication Year 1995-01,Volume 6,Issue #1,Page 19-25
- Journal Title
- CELL GROWTH & DIFFERENTIATION
- Publication Type
- Journal Article
- Abstract
- Rearrangement or translocation of the SCL gene is the most common genetic abnormality observed in human T-cell acute lymphocytic leukemia and results in the aberrant expression of SCL. To examine the oncogenic potential of this gene, an SCL-retrovirus was used to infect mouse bone marrow cells, which were then used to reconstitute C57/BL6 mice. Expression of SCL did not perturb the composition nor number of day 12 or day 13 colony forming unit-spleen. In total, 141 mice reconstituted with SCL-infected bone marrow and 103 control-mice were monitored for up to 2 years with no difference in survival, hematocrit, white cell count, or differential white cell count. As expected, from day 200 onwards, mice died due to radiation-induced thymomas; SCL provirus was not detected in these tumors. Thus, despite SCL being strongly implicated in the development of human leukemia, its enforced expression in mice using a retrovirus and bone marrow reconstitution was insufficient to generate leukemia.
- Publisher
- AMER ASSOC CANCER RESEARCH
- Keywords
- HEMATOPOIETIC STEM-CELLS; ACUTE LYMPHOBLASTIC-LEUKEMIA; CHRONIC MYELOGENOUS LEUKEMIA; COLONY-STIMULATING FACTOR; LOOP-HELIX GENE; MYELOPROLIFERATIVE SYNDROME; ERYTHROID-DIFFERENTIATION; CONDITIONED MEDIUM; MYELOID-LEUKEMIA; FORMING CELLS
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Creation Date: 1995-01-01 12:00:00