MEASUREMENT OF TSH RECEPTOR BLOCKING IMMUNOGLOBULINS USING H-3 ADENINE INCORPORATION INTO FRTL-5 AND JP09 CELLS - USE IN A CHILD WITH NEONATAL-HYPOTHYROIDISM
- Author(s)
- MICHELANGELI, VP; POON, CW; ARNUS, EE; FRAUMAN, AG; CONNELLY, J; Colman, PG;
- Details
- Publication Year 1995-01,Volume 42,Issue #1,Page 39-44
- Journal Title
- CLINICAL ENDOCRINOLOGY
- Publication Type
- Journal Article
- Abstract
- OBJECTIVE The aim of this study was to develop an assay for the measurement of thyroid blacking antibodies (TBAb), based on the ability of patient serum to inhibit TSH stimulated H-3-cAMP production following incubation of FRTL-5 or JPO9 cells with H-3-adenine. The assay was then used to evaluate a child born with neonatal hypothyroidism. DESIGN The levels of TBAb, TSAb (thyroid stimulating antibodies), TBII (TSH binding inhibitory antibodies), and the thyroid antibodies anti-thyroid peroxidase and thyroglobulin antibodies were measured in both mother and child over a 6-month post-natal period. PATIENT The assay for TBAb was used to evaluate a child born with neonatal hypothyroidism whose mother had a history of hypothyroidism due to Hashimoto's thyroiditis. A Tc-99m pertechnetate scan showed no evidence of functioning thyroid tissue. At 20 months of age an ultrasound verified a normally positioned thyroid. RESULTS Initially, high levels of TBII and antithyroid antibodies were present in the serum of both mother and child. In both, the levels of TSAb were undetectable but there were significant levels of TBAb. The levels of TBAb decreased to control levels in the child within 2 months of birth but remained elevated in the mother's serum. CONCLUSIONS This case of neonatal hypothyroidism associated with the passage of thyroid blocking antibodies demonstrates the utility of this new assay in the differential diagnosis of neonatal hypothyroidism.
- Publisher
- BLACKWELL SCIENCE LTD
- Keywords
- THYROID-STIMULATING IMMUNOGLOBULINS; SEQUENTIAL SERUM MEASUREMENTS; TRANSIENT HYPOTHYROIDISM; THYROTROPIN RECEPTOR; GRAVES-DISEASE; BINDING; INHIBITOR; AUTOANTIBODIES; ANTIBODIES; EXPRESSION
- Publisher's Version
- https://doi.org/10.1111/j.1365-2265.1995.tb02596.x
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1995-01-01 12:00:00